Several pairs of sneakers worn by famous athletes like Michael Johnson and Michael Jordan are among the lots offered.
On the same day the Olympics opens this month, Sotheby's will put on sale running shoes made by Nike's co-founder for 1960s Olympian Harry Jerome that could fetch $1 million.
Tim Berners-Lee's source code for the World Wide Web sold Wednesday for $5.4 million in the form of non-fungible token.
The auction house is seeing the boom in a formerly niche market as a way to attract younger clients into the world of auctions.
Sotheby's will offer in March a 1967 vintage bottle of the Macallan single-malt whisky, which was created in collaboration with British artist Sir Peter Blake.
Next month, Sotheby's will auction off a pair of Air Jordan worn by none other than Michael Jordan himself.
The painter's Abstraktes Bild (649-2) painting recently sold for US$27.7 million at Sotheby's "Contemporary Art" sale.
The autographed Air Jordan 1 Nike sneakers, made for Michael Jordan in 1985, has set a record for basketball shoes.
Online bidding will invigorate art auction houses big and small, in turning point for industry.